Article’s

Eco-friendly culture media have gained significant attention as sustainable alternatives to conventional synthetic media used in microbiology and biotechnology. The increasing cost of commercial media, environmental concerns, and the need for locally available resources have driven research toward plant-based substrates. Coconut sprout, a natural structure formed during coconut germination, is rich in carbohydrates, proteins, minerals, vitamins, and growth-promoting compounds. When processed into powder form, coconut sprout becomes a stable, easily handled raw material suitable for culture media formulation. This review summarises available literature related to coconut-derived media and highlights the potential of coconut sprout powder as an eco-friendly culture medium component. The article discusses the biological nature of the coconut sprout, its nutritional and biochemical composition, powder preparation techniques, and media formulation approaches. Applications in microbial culture and plant tissue culture are reviewed, emphasising sustainability, biodegradability, and cost-effectiveness. Challenges such as nutrient variability, standardisation, and storage stability are also addressed. Overall, coconut sprout powder represents a promising natural resource for developing low-cost and environmentally friendly culture media, particularly in resource-limited laboratories.