Article’s

HPTLC method includes selection of stationary phases, mobile phases, detection methods, and optimization of experimental conditions to achieve high resolution, sensitivity, and reproducibility. HPTLC is one type of planner chromatography and most advanced form of instrumental TLC. HPTLC operates on the same separation by adsorption concept as TLC. HPTLC offer wide choice of stationary phase Like Silica gel for normal phase and C8, C18 for reversed phase modes where sample can be detectable in nanograms with increased precision and sensitivity. HPTLC is a great instrument for detecting adulterations and is well suited for checking stability as well as assessing and tracking the processes of cultivation, harvesting, and extraction.Furthermore, the article explores the wide range of applications of HPTLC in pharmaceutical, environmental, food, and forensic analysis by integration of HPTLC with other analytical techniques, such as mass spectrometry (MS) and spectrophotometry, to enhance its capabilities.. This review aims to serve as a valuable resource for researchers and analytical chemists involved in the development and optimization of HPTLC methods for complex matrix. Keywords : HPTLC , Resolution , Advantage , Pharmaceutical